Figure 1: Suite2p pipeline. a, Example volume imaging data. b, X/Y motion correction (left: before, right: after). c, ROI detection (left) and trace extraction with neuropil extraction based on surrounding pixels (right). d, Classification of ROIs into somatic and non-somatic (i.e. dendrites and other neuropil). e, The graphical user interface (GUI) can be used for manual curation and results visualization. f, Multi-plane recording of over 100,000 neurons using a standard Thorlabs Bergamo2 scope (see Methods for exact configuration). Somatic ROIs for example plane shown in different colors. g, Neural activity raster from recording in f, with neurons sorted by Rastermap, colored by the corridor that the mouse is in [28].