Panel of data figures to illustrate comparative analysis of sex-specific gene expression evolution at inter-species comparison level in male and female house mice, including transcriptomic distance analysis and gene differentiation expression analysis. By analyzing extensive RNA-seq data from the house mouse complex across multiple organs and subspecies, this study reveals that male reproductive organs, especially the testis, show accelerated expression divergence. Testicular long non-coding RNAs evolve most rapidly, with differences arising within just thousands of years. In contrast, somatic tissues and female reproductive organs show minimal sex-specific evolutionary dynamics.