The Minimum Information Required for A Glycomics Experiment (MIRAGE) guidelines have established comprehensive reporting standards for glycomics research, yet their implementation in semantic web technologies remains limited. We present the first comprehensive semantic formalization of MIRAGE guidelines through an integrated RDF ontology framework comprising the MIRAGE Ontology and MIRAGE-DCAT3 vocabulary. The MIRAGE Ontology models glycan structures, biological specimens, analytical instruments, and experimental processes with formal OWL semantics and SHACL validation constraints. The complementary MIRAGE-DCAT3 vocabulary extends W3C DCAT3 with glycomics-specific metadata properties for dataset cataloging and discovery. Our implementation addresses critical challenges in glycomics data interoperability through comprehensive mappings to established ontologies including GlycoRDF, PSI-MS, and DCTERMS. This semantic framework enables automated quality assessment, federated data querying, and enhanced reproducibility in glycomics research, supporting broader adoption of FAIR principles in the glycobiology community. The framework demonstrates comprehensive coverage of MIRAGE reporting requirements across multiple analytical platforms including mass spectrometry, liquid chromatography, capillary electrophoresis, NMR spectroscopy, and lectin microarray analysis.

The Minimum Information Required for A Glycomics Experiment (MIRAGE) guidelines have established comprehensive reporting standards for glycomics research, yet their implementation in semantic web technologies remains limited. We present the first comprehensive semantic formalization of MIRAGE guidelines through an integrated RDF ontology framework comprising the MIRAGE Ontology and MIRAGE-DCAT3 vocabulary. The MIRAGE Ontology models glycan structures, biological specimens, analytical instruments, and experimental processes with formal OWL semantics and SHACL validation constraints. The complementary MIRAGE-DCAT3 vocabulary extends W3C DCAT3 with glycomics-specific metadata properties for dataset cataloging and discovery. Our implementation addresses critical challenges in glycomics data interoperability through comprehensive mappings to established ontologies including GlycoRDF, PSI-MS, and DCTERMS. This semantic framework enables automated quality assessment, federated data querying, and enhanced reproducibility in glycomics research, supporting broader adoption of FAIR principles in the glycobiology community. The framework demonstrates comprehensive coverage of MIRAGE reporting requirements across multiple analytical platforms including mass spectrometry, liquid chromatography, capillary electrophoresis, NMR spectroscopy, and lectin microarray analysis.